Chapter 1: mRNA Fundamentals
What Is mRNA?
Messenger RNA (mRNA) is a single-stranded nucleic acid that carries genetic information from DNA to ribosomes.
- Built from ribonucleotides (A, U, C, G)
- Synthesized during transcription
- Read during translation to build proteins
Key idea: mRNA is the temporary, editable copy of genetic instructions.
mRNA in the Central Dogma
DNA stores information; mRNA transmits it.
- DNA: stable, double-stranded archive
- mRNA: short-lived working copy
- rRNA: forms ribosome structure
- tRNA: brings amino acids
Flow of information:
> DNA → mRNA → Protein
Disrupting any step can alter protein levels and cell behavior.
Structure of Eukaryotic mRNA
Key features:
- 5′ cap: modified G; protects and helps ribosome binding
- 5′ UTR: regulates translation initiation
- Coding sequence (ORF): triplet codons specifying amino acids
- 3′ UTR: contains regulatory elements
- Poly(A) tail: many A’s; stabilizes, aids export, translation
Different regions enable fine control of protein production.
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